Journal: Advanced Science

Article Title: Melanoma Derived Exosomes Amplify Radiotherapy Induced Abscopal Effect via IRF7/I‐IFN Axis in Macrophages

doi: 10.1002/advs.202304991

Figure Lengend Snippet: CircPIK3R3/IRF7/I‐IFN axis participates in the combination of radiotherapy and anti‐PD1 mediated abscopal effect. A) The treatment model involving RT, anti‐PD1, and RO8191: C57/BL6 mice were subcutaneously inoculated with 1 × 106 sh‐NC B16F10 cells or sh‐circ‐0011074 B16F10 cells. On day 5, C57/BL6 mice were intravenously injected with 1 × 106 B16F10‐luc cells. Starting from day 6, mice were administered the IFN receptor agonist RO8191 via daily intraperitoneal injections at a dose of 1 mg kg −1 . On day 7, radiotherapy was initiated, with a daily dose of 8 Gy administered for 3 consecutive days. On day 7, mice were also administered anti‐PD1 via intraperitoneal injection every 2 days at a dose of 100 µg/mouse until the endpoint of observation. B,C) Measurement of subcutaneous tumor weight in each group to assess treatment efficacy ( n = 3). D,E) Evaluation of fluorescent intensity in lung metastatic foci using bioluminescence imaging to assess treatment efficacy ( n = 6). F,G) Immunohistochemical examination of CD8 + T cell infiltration in subcutaneous tumors and lung metastatic foci ( n = 3). H,I) Immunofluorescence detection of IRF7 + macrophage infiltration in subcutaneous tumors and lung metastatic foci (n = 3). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by two‐tailed unpaired Student t‐test.

Article Snippet: Anti‐mouse PD‐1 mAb (Bio X Cell, USA) was administered intraperitoneally (200 mg per mouse) every 2 days during treatment.

Techniques: Injection, Imaging, Immunohistochemical staining, Immunofluorescence, Two Tailed Test

Journal: Biomedicines

Article Title: Muscle and Muscle-like Autoantigen Expression in Myasthenia Gravis Thymus: Possible Molecular Hint for Autosensitization.

doi: 10.3390/biomedicines11030732

Figure Lengend Snippet: Figure 5. HSP60 expression in MG and control thymuses. (a–d) HSP60 mRNA levels in normal thy- muses (CTR), follicular hyperplastic (MG FH) thymuses from MG patients stratified in corticosteroid- naïve (MG FH No Cortico) and -treated (MG FH Cortico), thymomas from non-MG patients (NON- MG T), thymomas (MG T) from MG patients stratified in corticosteroid-naïve (MG T No Cortico) and -treated (MG T Cortico), and NON-MG T and MG T stratified according with WHO thymoma type. In the graphs, gene expression levels were expressed as relative values (2−∆Ct × 100) normalized toward the endogenous 18S. Mann–Whitney test, * p < 0.05; ** p < 0.01; and *** p < 0.001.

Article Snippet: Antibodies to human HSP60 were quantified in serum from 27 AChR-positive and 19 seronegative MG patients and 14 healthy controls, using the Human Hsp60 Autoantibody ELISA Kit (Novus biologicals, Bio-techne SRL, Milan, Italy).

Techniques: Expressing, Control, Gene Expression, MANN-WHITNEY